Acute toxicity of beta gamma-CAT, a naturally existing non-lens beta gamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions
机构:[1]Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, PR China[2]Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, PR China外科科室麻醉手术科(医技)昆明医科大学附属第一医院云南省第一人民医院[3]Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China
In vertebrates, non-lens beta gamma-crystallins are widely expressed in various tissues, but their functions are unknown. The molecular mechanisms of trefoil factors, initiators of mucosal healing and being greatly involved in tumorigenesis, have remained elusive. beta gamma-CAT is the first example of a naturally existing multifunctional protein complex of a non-lens beta gamma-crystallin and a trefoil factor from frog Bombina maxima skin secretions. Here we report the investigation of its in vivo toxic effects on mice and rabbits. The LD(50) values of beta gamma-CAT on mice were determined to be 0.4 mg/kg and 20 mu g/kg under intraperitoneal (i.p.) and intravenous (i.v.) injection, respectively. The mice subcutaneously injected with beta gamma-CAT (6 mu g/g body weight) showed strong hyperaemia of subcutaneous capillary vessel, but no hemorrhagic spots were observed. Intravenous injection of beta gamma-CAT in rabbits (8-22 mu g/kg body weight) caused a rapidly hypotensive effect and followed with cardiovascular collapse. Injection with beta gamma-CAT (22 mu g/kg, i.v.) significantly decreased hematocrit (P < 0.05) and mean corpuscular volume (P < 0.05) of the rabbits in 5 min. At the same time, the counts of platelets and white blood cells were significantly decreased (P < 0.05), while the blood levels of glucose, lactate dehydrogenase and serum glutamic-oxaloacetic transaminase were significantly increased (P < 0.05). Furthermore, serials of tissues edema and damages were also observed. These results indicate that beta gamma-CAT rapidly caused several in vivo toxic effects on mammals and its lethal toxic potency was mainly contributed by hypotension and cardiovascular collapse, providing new clues for the understanding of the patho-physiological roles of non-lens beta gamma-crystallins and trefoil factors. (C) 2008 Elsevier Ltd. All rights reserved.
基金:
The Chinese National Natural Science FoundationNational Natural Science Foundation of China [30630014, 30570359]; Chinese Academy of SciencesChinese Academy of Sciences; The Yunnan Science and Technology Commission [2006PT08-1]
第一作者机构:[1]Biotoxin Units, Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, PR China[2]Department of Anesthesiology, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, PR China[3]Graduate School of the Chinese Academy of Sciences, Beijing 100039, PR China
共同第一作者:
通讯作者:
通讯机构:[*1]Kunming Institute of Zoology, Chinese Academy of Sciences, 32 East Jiao Chang Road, Kunming 650223, Yunnan, PR China.
推荐引用方式(GB/T 7714):
Qian Jin-Qiao,Liu Shu-Bai,He Ying-Ying,et al.Acute toxicity of beta gamma-CAT, a naturally existing non-lens beta gamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions[J].TOXICON.2008,52(1):22-31.doi:10.1016/j.toxicon.2008.05.007.
APA:
Qian, Jin-Qiao,Liu, Shu-Bai,He, Ying-Ying,Lee, Wen-Hui&Zhang, Yun.(2008).Acute toxicity of beta gamma-CAT, a naturally existing non-lens beta gamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions.TOXICON,52,(1)
MLA:
Qian, Jin-Qiao,et al."Acute toxicity of beta gamma-CAT, a naturally existing non-lens beta gamma-crystallin and trefoil factor complex from frog Bombina maxima skin secretions".TOXICON 52..1(2008):22-31