机构：[a]College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China[b]School of Life Science and Biotechnology, Dalian University of Technology, Dalian, Liaoning 116024, China[c]Biotoxin Units of Key Laboratory of Animal Models and Human Disease Mechanisms, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China[d]Yunnan Clinical Research Center of Breast Cancer, The Third Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China[e]Clinical Laboratory, The First Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China昆明医科大学附属第一医院云南省第一人民医院
Amphibian opiate peptides including dermorphins and deltorpins have been recently found only in the skin of South American frogs belonging to the subfamily Phyllomedusinae (Phyllomedusa, Agalychnis and Pachymedusa species). No opiate peptides have ever been identified from other amphibians or organs except skin. Here we report the purification and characterization of a novel antinociceptive peptide named odorranaopin from the homogenates of the frog brains, Odorrana grahami, which is also the first antinociceptive peptide found in Ranidae amphibian. Odorranaopin comprises 17 amino acid residues with the sequence of DYTIRTRLHQESSRKVL (Mr 2102 Da). The cDNA encoding odorranaopin was cloned from the frog brain cDNA library, and it was confirmed to be a specific gene. The odorranaopin precursor deduced is composed of 61 amino acid residues including the predicted signal peptide, acidic spacer peptide and mature odorranaopin positioned at the C-terminus. Odorranaopin could inhibit nociceptive responses induced by formalin and acetic acid. It also inhibited the contractile responses of ileum smooth muscle induced by bradykinin, implying that the antinociceptive activity of odorranaopin possibly results from its blockade on bradykinin or bradykinin receptor functions. Odorranaopin is the first antinociceptive peptide found in Ranidae amphibian. (C) 2011 Elsevier Masson SAS. All rights reserved.
基金：
This work was supported by
the grants from Chinese National Natural Science Foundation
(30900240, 41076098).
第一作者机构：[a]College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China[d]Yunnan Clinical Research Center of Breast Cancer, The Third Affiliated Hospital of Kunming Medical College, Kunming 650032, Yunnan, China
共同第一作者：
通讯作者：
通讯机构：[*1]College of Life Sciences, Hebei Normal University, Shijiazhuang, Hebei 050016, China.
推荐引用方式(GB/T 7714)：
Wenlin Chen,Xuening Yang,Lingling Chen,et al.Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami[J].BIOCHIMIE.2011,93(7):1110-1114.doi:10.1016/j.biochi.2011.03.007.
APA：
Wenlin Chen,Xuening Yang,Lingling Chen,Xiaolong Yang,Feifei Feng...&Haining Yu.(2011).Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami.BIOCHIMIE,93,(7)
MLA：
Wenlin Chen,et al."Gene cloning and characterization of novel antinociceptive peptide from the brain of the frog, Odorrana grahami".BIOCHIMIE 93..7(2011):1110-1114
