资源类型：
期刊
收录情况：
◇ 统计源期刊
◇ 北大核心
◇ CSCD-C
◇ 中华系列
文章类型：
论著
机构：
[1]First Peoples' Hospital of Kunming, Affiliated Ganmei Hospital, Kunming Medical University, Kunming 650011, China
出处：
National Medical Journal of China.2011,91(14):
ISSN：
0376-2491
关键词：
Breast noeplasms
DNA
mitochondrial
Genome
Somatic mutation
摘要：
Objective: To investigate the correlation of somatic mutations in whole genome of mitochondrial DNA (mtDNA) in patients with breast tumor. Methods: The DNA of tumor tissue and peripheral blood in 4 benign breast tumor patients from August 2009 to December 2009 in our hospital were extracted. The mtDNA whole genomes were amplified by polymerase chain reaction (PCR) and the mutations of products screened by sequencing to compare the difference of mutation distribution between tumor tissue and peripheral blood. The likelihood of somatic mutations in tumor tissue was determined. Results: The mutation spectrum of mtDNA genomes was obtained by PCR and sequencing. Then a phylogenetic tree was constructed to identify their haplogroups (D4i, G1, R9b, N9a). Their private mutations in peripheral blood were detected and the somatic mutation at 16292 position was found in one patient (haplogroups: R9b). Conclusion: Based on the extensively study on the mtDNA genomes from the tumor issues of 4 patients, our current report observed only a single somatic variation from the control region, no any further mutations with potential function from the coding region were observed. Copyright © 2011 by the Chinese Medical Association.
第一作者：
Yang, Y
第一作者机构：
[1]First Peoples' Hospital of Kunming, Affiliated Ganmei Hospital, Kunming Medical University, Kunming 650011, China
通讯作者：
Li, L
推荐引用方式(GB/T 7714)：
Yang Y,He J.-D,Guo X.-J,等.Correlation of breast tumor pathogenesis and somatic mutations on the basis of mitochondrial DNA whole genome[J].National Medical Journal of China.2011,91(14):
