机构:[1]Departments of Thoracic Surgery, Yan’an Affiliated Hospital of Kunming Medical University, 245 East Renmin Road, Kunming 650051, China[2]Departments of Gastroenterology, Yan’an Affiliated Hospital of Kunming Medical University, 245 East Renmin Road, Kunming 650051, China内科科室外科科室消化内科普通外科普通外一科(胃肠与疝外科)[3]The First Affiliated Hospital of Kunming Medical University, 295 Xichang Road, Kunming 650032, China昆明医科大学附属第一医院妇科妇产科外科科室[4]Department of Thoracic Surgery, Jiujiang University Clinical Medical College, 57 East Xunyang Road, Jiujiang 332000, China[5]Department of Cell Biology and Medical Genetics, Kunming Medical University, Kunming 650050, China.
The NUP58 gene encodes a nucleus-pore protein that is a component of nuclear pore complex (NPC). NPC facilitates the transportation of macromolecules (ions and other substances) into the nuclei of eukaryotic cells. However, there are no relevant reports about the NUP58 gene in human lung cancer. In this study, we demonstrated that NUP58 was highly expressed in the primary and metastatic foci of lung adenocarcinoma, with low expression in adjacent tissues and normal lung tissue. In patients with lung adenocarcinoma, the NUP58 gene was highly expressed in patients with stage IV disease (P < 0.05); NUP58 knockdown using a lentiviral vector-mediated shRNA inhibited metastasis and invasion of lung adenocarcinoma cell lines A549 and H1299 in vivo and in vitro. Furthermore, silencing of NUP58 resulted in altered expression of EMT markers, associated GSK-3β/Snail pathways, tumor metastasis and invasion factors. In conclusion, these findings demonstrated that NUP58 can promote the metastasis and invasion of lung adenocarcinoma, which can be partially attributed to the GSK-3β/Snail signaling pathway.
基金:
The present study was supported by the Health
and Family Planning Commission of Yunnan
Province (2016IV5315).
第一作者机构:[1]Departments of Thoracic Surgery, Yan’an Affiliated Hospital of Kunming Medical University, 245 East Renmin Road, Kunming 650051, China[*1]Department of Thoracic Surgery, Yan’an Affiliated Hospital of Kunming Medical University, 245 East Renmin Road, Kunming 650051, China.
共同第一作者:
通讯作者:
通讯机构:[1]Departments of Thoracic Surgery, Yan’an Affiliated Hospital of Kunming Medical University, 245 East Renmin Road, Kunming 650051, China[*1]Department of Thoracic Surgery, Yan’an Affiliated Hospital of Kunming Medical University, 245 East Renmin Road, Kunming 650051, China.
推荐引用方式(GB/T 7714):
Jianlin Shi,Chen Li,Hui Wang,et al.NUP58 facilitates metastasis and epithelial-mesenchymal transition of lung adenocarcinoma via the GSK-3β/Snail signaling pathway.[J].American journal of translational research.2019,11(1):393-405.
APA:
Jianlin Shi,Chen Li,Hui Wang,Benshan Xiao&Wanfang Qiu.(2019).NUP58 facilitates metastasis and epithelial-mesenchymal transition of lung adenocarcinoma via the GSK-3β/Snail signaling pathway..American journal of translational research,11,(1)
MLA:
Jianlin Shi,et al."NUP58 facilitates metastasis and epithelial-mesenchymal transition of lung adenocarcinoma via the GSK-3β/Snail signaling pathway.".American journal of translational research 11..1(2019):393-405