Diabetic nephropathy (DN) is a common chronic complication of diabetes. In this study, we aimed to explore the potential role of lncRNA LINC-00162 in the pathogenic process of DN. LncRNA microarray analysis, real-time PCR, IHC computational analysis and luciferase assay were performed to explore the regulatory relationship among LINC00162, miR-383 and HDAC9. There was an obvious difference between T2D + DN and T2D - DN patients in their levels of eGRF and albuminuria. A significant difference was observed between T2D + DN and T2D - DN groups in terms of their LINC00162 expression. In particular, LINC00162 and HDAC9 were highly expressed, while miR-383 was lowly expressed in tissues derived from the T2D + DN group compared with those in tissues derived from the T2D - DN group. MiR-383 was able to bind to LINC00162, while HDAC9 was a direct downstream target of miR-383 with a complementary miR-383 binding site located in the 3 ' UTR of HDAC9. LINC00162 reduced miR-383 expression and further up-regulated HDAC9 expression, while miR-383 mimics reduced HDAC9 expression under a dose-dependent manner. In summary, we suggested for the first time that down-regulation of LINC00162 was associated with the development of DN in T2D via the up-regulation of miR-383 expression and reduction of HDAC9 expression.
基金：
Yunnan Applied Basic Research Projects - Union Foundation [2018FE001(-032), 2017FE467(-043)]
第一作者机构：[1]Kunming Med Univ, Dept Nephrol, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China[2]Kunming Med Univ, Yunnan Key Lab Lab Med, Affiliated Hosp 1, Kunming, Yunnan, Peoples R China
通讯作者：
通讯机构：[7]Department of Gastrointestinal Surgery, Institute of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, No.295, Xichang Road, Kunming 650032, Yunnan, China[8]Department of Nephrology, West China Hospital, Sichuan University, No. 37, Guoxue Alley, Chengdu 610041, Sichuan, China
推荐引用方式(GB/T 7714)：
Fan WenXing,Wen XiaoLing,Zheng JinFeng,et al.LINC00162 participates in the pathogenesis of diabetic nephropathy via modulating the miR-383/HDAC9 signalling pathway[J].ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY.2020,48(1):1047-1054.doi:10.1080/21691401.2020.1773487.
APA：
Fan, WenXing,Wen, XiaoLing,Zheng, JinFeng,Wang, KunHua,Qiu, HongYu...&Su, Feng.(2020).LINC00162 participates in the pathogenesis of diabetic nephropathy via modulating the miR-383/HDAC9 signalling pathway.ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY,48,(1)
MLA：
Fan, WenXing,et al."LINC00162 participates in the pathogenesis of diabetic nephropathy via modulating the miR-383/HDAC9 signalling pathway".ARTIFICIAL CELLS NANOMEDICINE AND BIOTECHNOLOGY 48..1(2020):1047-1054
