机构：[1]Experiment Center of Basic Medical Sciences of Kunming Medical University, Kunming City, P. R. China[2]Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming City, P. R. China外科科室骨科昆明医科大学附属第一医院
Introduction MiR-200c plays a central role in glucose metabolism in cancer cells. However, its upstream regulators in this process are unknown. CircRNA CSPP1 (circCSPP1) was predicted to bind to premature miR-200c, an oncogenic miRNA. Therefore, we explored their interaction in osteosarcoma (OS). Methods Differential circCSPP1 and miR-200c expression in OS was analyzed using RT-qPCR. Glucose metabolism was analyzed by glucose uptake assay. Subcellular circCSPP1 location in OS cells was detected using cellular fractionation assay. The direct interaction between circCSPP1 and miR-200c was explored using RNA-RNA pull-down assay. The role of circCSPP1 in miR-200c maturation was investigated by analyzing both mature and premature miR-200c levels in OS cells with circCSPP1 overexpression. Results CircCSPP1 and premature miR-200c levels were increased while mature miR-200c level was decreased in OS. CircCSPP1 was detected in both the nuclear and cytoplasm fractions of OS cells. CircCSPP1 directly interacted with premature miR-200c. CircCSPP1 overexpression increased premature miR-200c level, glucose uptake, and cell proliferation, but decreased mature miR-200c level. MiR-200c overexpression suppressed the role of circCSPP1 in OS cells. Conclusions CircCSPP1 promotes OS cell proliferation and increases glucose metabolism by suppressing miR-200c maturation.
基金：
This
study was supported by Yunnan Provincial Bone and Joint Disease
Clinical Medicine Center Project (No. ZX2019-03-04).
第一作者机构：[1]Experiment Center of Basic Medical Sciences of Kunming Medical University, Kunming City, P. R. China
通讯作者：
通讯机构：[2]Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, Kunming City, P. R. China[*1]Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, No.295 Xichang Road, Kunming City, Yunnan Province 650031, P. R. China.[*2]Department of Orthopedics, The First Affiliated Hospital of Kunming Medical University, No.295 Xichang Road, Kunming City, Yunnan Province 650031, P. R. China.
推荐引用方式(GB/T 7714)：
Zhang L.,Yang S. T.,Wang C.,et al.Circle RNA circCSPP1 promotes human osteosarcoma cell proliferation and increases glucose metabolism by suppressing miR-200c maturation[J].HUMAN & EXPERIMENTAL TOXICOLOGY.2022,41:doi:10.1177/09603271221097364.
APA：
Zhang, L.,Yang, S. T.,Wang, C.,Zhang, L. C.,Zhang, X....&Ma, K..(2022).Circle RNA circCSPP1 promotes human osteosarcoma cell proliferation and increases glucose metabolism by suppressing miR-200c maturation.HUMAN & EXPERIMENTAL TOXICOLOGY,41,
MLA：
Zhang, L.,et al."Circle RNA circCSPP1 promotes human osteosarcoma cell proliferation and increases glucose metabolism by suppressing miR-200c maturation".HUMAN & EXPERIMENTAL TOXICOLOGY 41.(2022)
