Isobaric tags for relative and absolute quantification-based proteomic analysis of testis biopsies in rhesus monkeys treated with transient scrotal hyperthermia
机构:[1]Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China[2]Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming, China外科科室医技科室生殖遗传科医学影像中心CT室昆明医科大学附属第一医院[3]Wuhan Women and Children's Health Care Center of Hubei Province, Wuhan, China[4]Changsha Hospital for Maternal and Child Health Care, Changsha, China[5]Reproductive Medicine Center, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China
This study aimed to examine the cellular and molecular events that occur in rhesus monkey testes after scrotal hyperthermia. Eight male adult rhesus monkeys were subjected to scrotal hyperthermia at 43 degrees C for 30 min daily for 6 consecutive days. Sperm concentration, reproductive hormones, and testis histology were examined before hyperthermia (day 0), and at 8, 15, 30, 45, 60, 75, and 90 days after the initiation of hyperthermia. iTRAQ-based proteomic analysis was conducted on testicular tissues collected on days 0, 8, and 60 to identify differentially expressed proteins at the early and recovery stages of testicular damage. The sperm concentration was significantly decreased at days 30 and 45 after treatment (p < 0.01) and recovered to baseline at day 60. When compared with day 0, 101 and 24 differentially expressed proteins were identified at days 8 and 60 after heat treatment, respectively. The molecular functions of the differentially expressed proteins at day 8 were mainly nucleic acid binding, unfolded protein binding, nucleotide binding, and nucleoside phosphate binding. Spliceosome was enriched as the most significant pathway at day 8. CIRBP, PSIP1, Sam68, and Decorin were validated and found to be consistent with the proteomic data, indicating the reliability of the proteomic profiles identified in this study. In summary, we suggest that the proteins identified in this study may play important roles in heat-induced spermatogenic impairment. Some of these proteins, such as CIRBP, PSIP1, Sam68, and Decorin, may be early molecular targets responsible for spermatogenesis suppression induced by heat treatment.
基金:
National Key Research and Development Program of China [2016YFC1000903]; National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81671507]; Independent Innovation funding of Huazhong University of Science and Technology [2016YXMS164]; Health and Family Planning Commission Program of Hubei Province [WJ2017M056]
第一作者机构:[1]Family Planning Research Institute, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, China[2]Department of Reproduction and Genetics, The First Affiliated Hospital of Kunming Medical University, Kunming, China
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推荐引用方式(GB/T 7714):
Rao Meng,Ma Sha,Hu Shifu,et al.Isobaric tags for relative and absolute quantification-based proteomic analysis of testis biopsies in rhesus monkeys treated with transient scrotal hyperthermia[J].ONCOTARGET.2017,8(49):85909-85925.doi:10.18632/oncotarget.20719.
APA:
Rao, Meng,Ma, Sha,Hu, Shifu,Lei, Hui,Wu, Yanqing...&Zhu, Changhong.(2017).Isobaric tags for relative and absolute quantification-based proteomic analysis of testis biopsies in rhesus monkeys treated with transient scrotal hyperthermia.ONCOTARGET,8,(49)
MLA:
Rao, Meng,et al."Isobaric tags for relative and absolute quantification-based proteomic analysis of testis biopsies in rhesus monkeys treated with transient scrotal hyperthermia".ONCOTARGET 8..49(2017):85909-85925