机构：[1]Department of Pathogen Biology and Immunology, Kunming Medical University, Kunming, Yunnan Province, China[2]Department of Infectious Diseases, The First Affiliated Hospital, Kunming Medical University, Kunming, Yunnan Province, ChinacBiomedical Engineering Research Center, Kunming Medical University, Kunming, Yunnan Province, China昆明医科大学附属第一医院[3]Department of Public Health, Kunming Medical University, Kunming, Yunnan Province, China[4]Department of Pathology, Kunming Medical University, Kunming, Yunnan Province, China[5]Department of Biochemistry, Kunming Medical University, Kunming, Yunnan Province, China[6]Eukaryotic Pathogens Innovation Center, Department of Genetics and Biochemistry, Clemson University, SC, USA[7]Department of Entomology, Pennsylvania State University, University Park, PA, USA
The conventional method of estimating parasite densities employ an assumption of 8000 white blood cells (WBCs)/mu l. However, due to leucopenia in malaria patients, this number appears to overestimate parasite densities. In this study, we assessed the accuracy of parasite density estimated using this assumed WBC count in eastern Myanmar, where Plasmodium vivax has become increasingly prevalent. From 256 patients with uncomplicated P. vivax malaria, we estimated parasite density and counted WBCs by using an automated blood cell counter. It was found that WBC counts were not significantly different between patients of different gender, axillary temperature, and body mass index levels, whereas they were significantly different between age groups of patients and the time points of measurement. The median parasite densities calculated with the actual WBC counts (1903/mu l) and the assumed WBC count of 8000/mu l (2570/mu l) were significantly different. We demonstrated that using the assumed WBC count of 8000 cells/mu l to estimate parasite densities of P. vivax malaria patients in this area would lead to an overestimation. For P. vivax patients aged five years and older, an assumed WBC count of 5500/mu l best estimated parasite densities. This study provides more realistic assumed WBC counts for estimating parasite densities in P. vivax patients from low-endemicity areas of Southeast Asia. (C) 2016 Elsevier B.V. All rights reserved.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [U1202226, 31260508]; Special Research Funds from the Ministry of Education of China [20125317110001]; talent introduction project of Yunnan province [2013HA026]; National Institutes of Health, USAUnited States Department of Health & Human ServicesNational Institutes of Health (NIH) - USA [U19AI089672]; Academic Newcomer Scholarship from Yunnan province
第一作者机构：[1]Department of Pathogen Biology and Immunology, Kunming Medical University, Kunming, Yunnan Province, China[2]Department of Infectious Diseases, The First Affiliated Hospital, Kunming Medical University, Kunming, Yunnan Province, ChinacBiomedical Engineering Research Center, Kunming Medical University, Kunming, Yunnan Province, China
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推荐引用方式(GB/T 7714)：
Liu Huaie,Feng Guohua,Zeng Weilin,et al.A more appropriate white blood cell count for estimating malaria parasite density in Plasmodium vivax patients in northeastern Myanmar[J].ACTA TROPICA.2016,156:152-156.doi:10.1016/j.actatropica.2016.01.018.
APA：
Liu, Huaie,Feng, Guohua,Zeng, Weilin,Li, Xiaomei,Bai, Yao...&Cui, Liwang.(2016).A more appropriate white blood cell count for estimating malaria parasite density in Plasmodium vivax patients in northeastern Myanmar.ACTA TROPICA,156,
MLA：
Liu, Huaie,et al."A more appropriate white blood cell count for estimating malaria parasite density in Plasmodium vivax patients in northeastern Myanmar".ACTA TROPICA 156.(2016):152-156
