机构：[1]School of Medicine, Yunnan University, Kunming, Yunnan, China[2]Institute of Basic Science, Chinese Academy of Medical Sciences & PekingUnion Medical College, Beijing, China[3]The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan,China昆明医科大学附属第一医院
As an endogenous cytoprotective factor, the protection of estrogen and heat shock protein-70 (Hsp70) on DNA has been documented, respectively, but the functional interaction between estrogen and Hsp70 on DNA damage repair is largely unknown. We therefore investigated the relation between estrogen and Hsp70 in terms of DNA protection in in vitro. The findings showed a significant reduction in cell survival and elevation in oxidative stress while cells were exposed to amyloid beta (A beta(25-35)) peptide, but preincubation of the cells with 17 beta-estradiol (17 beta-E2) ameliorated this situation. In addition, 17 beta-E2 alleviated oxidized DNA damage induced by A beta and elevated the expression of Hsp70. However, the beneficial properties of 17 beta-E2 on reducing DNA damage were attenuated when Hsp70 gene was silenced accordingly. These results indicate that Hsp70 plays a role in DNA protection mediated by estrogen, and the DNA protection may be involved in Alzheimer's disease preventive effect from estrogen.
基金：
National 973 Fundamental Project of ChinaNational Basic Research Program of China [2010CB934002]; Yunnan Provincial Science and Technology Department [2011FZ005]
第一作者机构：[1]School of Medicine, Yunnan University, Kunming, Yunnan, China[2]Institute of Basic Science, Chinese Academy of Medical Sciences & PekingUnion Medical College, Beijing, China
通讯作者：
通讯机构：[*1]Department of Pharmacology, Institute of Basic Science, Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing 100005, China
推荐引用方式(GB/T 7714)：
Yilong Dong,Yanmei Wang,Haijing Yu,et al.Involvement of Heat Shock Protein 70 in the DNA Protective Effect From Estrogen[J].AMERICAN JOURNAL OF ALZHEIMERS DISEASE AND OTHER DEMENTIAS.2013,28(3):269-277.doi:10.1177/1533317513481096.
APA：
Yilong Dong,Yanmei Wang,Haijing Yu,Yanyong Liu,Nan Yang&Pingping Zuo.(2013).Involvement of Heat Shock Protein 70 in the DNA Protective Effect From Estrogen.AMERICAN JOURNAL OF ALZHEIMERS DISEASE AND OTHER DEMENTIAS,28,(3)
MLA：
Yilong Dong,et al."Involvement of Heat Shock Protein 70 in the DNA Protective Effect From Estrogen".AMERICAN JOURNAL OF ALZHEIMERS DISEASE AND OTHER DEMENTIAS 28..3(2013):269-277
