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TAT-Apoptin induces apoptosis in the human bladder cancer EJ cell line and regulates Bax, Bcl-2, caspase-3 and survivin expression

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收录情况： ◇ SCIE

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机构： [1]Department of Nephrology, The First Affiliated Hospital, Kunming Medical University, Kunming 650031 [2]Department of Urology, The Second Affiliated Hospital, Kunming Medical University, Kunming 650101, P.R. China

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关键词： TAT-Apoptin EJ cell line inhibition apoptosis expression

摘要：

In order to identify the antitumor effect of TAT-Apoptin on the human bladder cancer EJ cell line and study its impact on the expression of the apoptosis-related genes bax, bcl-2, caspase-3 and survivin, the MTT assay, real-time quantitative PCR and western blot analysis were used in this study. The results of the MTT assay indicated that TAT-Apoptin was able to inhibit the proliferation of EJ cells in a dose-dependent manner. The expression of Bax, Bcl-2, Caspase-3 and Survivin mRNA and protein following the treatment of the EJ cells with TAT-Apoptin (0.1, 0.5, 1, 10,50 and 100 mu g/ml) for 24,48 and 72 h was analyzed. Cell proliferation was significantly different after treatment with the various concentrations of TAT-Apoptin and the durations of treatment. The level of expression of Bcl-2 and Survivin in EJ cells decreased significantly, while that of Bax and Caspase-3 increased significantly at the mRNA and protein levels.

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出版当年[2013]版：

大类 | 4 区医学

小类 | 4 区医学：研究与实验

最新[2023]版：

大类 | 4 区医学

小类 | 4 区医学：研究与实验

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出版当年[2012]版：

Q4 MEDICINE, RESEARCH & EXPERIMENTAL

最新[2023]版：

Q3 MEDICINE, RESEARCH & EXPERIMENTAL

影响因子： 2.4 最新[2023版] 2.3 最新五年平均 0.344 出版当年[2012版] 0.344 出版当年五年平均 0.206 出版前一年[2011版] 0.941 出版后一年[2013版]

第一作者：

第一作者机构： [1]Department of Nephrology, The First Affiliated Hospital, Kunming Medical University, Kunming 650031

通讯作者：

通讯机构： [*1]Department of Urology, The Second Affiliated Hospital, Kunming Medical University, Kunming 650101, Yunnan, P.R. China

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