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Analysis of circular RNA expression profiles of lung cancer in Xuanwei, China(Open Access)

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机构: [a]Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming, China [b]Yunnan Key Laboratory of Laboratory Medicine, Kunming, China [c]Yunnan Institute of Laboratory Diagnosis, Kunming, China [d]Innovation Team of Yunnan Provincial Clinical Laboratory and Diagnosis, Kunming, China
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关键词: biomarker circRNA microarray circular RNA LCXW microRNA

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Background: Mounting evidence indicates that circular RNAs (circRNAs) could play a pivotal role in cancers. However, due to the lack of sensitive biomarkers, most lung cancer in Xuanwei (LCXW) patients are still diagnosed at an advanced stage accompany with distant metastasis. Methods: According to the stage of LCXW patients and tissue sources, circRNAs microarray detection was carried out in six groups. Considering fold change, raw intensity, the length of circRNAs, and P-value, we selected eight circRNAs for further study. A total of 50 paired LCXW tissues were carried out real-time quantitative polymerase chain reaction (RT-qPCR) in order to extended sample size to verify the expression of these circRNAs. Results: We designed 13 617 human circRNA probes for the human circular RNA microarray, detected 10 819 circRNA in six groups of samples; 537 circRNAs were differentially expressed consistently in every stage. Through RT-qPCR, we selected 8 circRNAs, three of which were upregulated (hsa_circ_0005927, hsa_circ_0069397 and hsa_circ_0000937) and five were downregulated (hsa_circ_0001936, hsa_circ_0005255, hsa_circRNA_406010, hsa_circ_0007064, hsa_circ_0000907) in tumor tissues, only hsa_circ_0001936 showed the opposite expression between microarray and RT-qPCR, others were consistent. Additionally, hsa_circ_0005927 and hsa_circ_0001936 were significantly correlated with tumor size, and hsa_circRNA_406010 was related to the prognosis of LCXW patients. Conclusion: Together, these results suggest that hsa_circ_0005927, hsa_circ_0001936, and hsa_circRNA_406010 may serve as the novel potential biomarkers for LCXW. Moreover, these results may provide a new insight for the pathogenesis of LCXW. © 2020 The Authors. Journal of Clinical Laboratory Analysis Published by Wiley Periodicals LLC.

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出版当年[2021]版:
大类 | 4 区 医学
小类 | 4 区 医学实验技术
最新[2023]版:
大类 | 4 区 医学
小类 | 3 区 医学实验技术
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出版当年[2020]版:
Q3 MEDICAL LABORATORY TECHNOLOGY
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Q2 MEDICAL LABORATORY TECHNOLOGY

影响因子: 最新[2023版] 最新五年平均 出版当年[2020版] 出版当年五年平均 出版前一年[2019版] 出版后一年[2021版]

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第一作者机构: [a]Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming, China [b]Yunnan Key Laboratory of Laboratory Medicine, Kunming, China [c]Yunnan Institute of Laboratory Diagnosis, Kunming, China [d]Innovation Team of Yunnan Provincial Clinical Laboratory and Diagnosis, Kunming, China
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通讯机构: [a]Department of Clinical Laboratory, First Affiliated Hospital of Kunming Medical University, Kunming, China [b]Yunnan Key Laboratory of Laboratory Medicine, Kunming, China [c]Yunnan Institute of Laboratory Diagnosis, Kunming, China [d]Innovation Team of Yunnan Provincial Clinical Laboratory and Diagnosis, Kunming, China
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