PRMT1 promotes extracellular matrix degradation and apoptosis of chondrocytes in temporomandibular joint osteoarthritis via the AKT/ FOXO1 signaling pathway
机构：[1]Department of Periodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China[2]Yunnan Key Laboratory of Stomatology, Kunming 650500, Yunnan Province, China[3]The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China昆明医科大学附属第一医院[4]Department of Orthodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China[5]The First Dental Clinic of the Affiliated Stomatology Hospital of Kunming Medical University, Kunming 650221, Yunnan Province, China
Temporomandibular joint osteoarthritis (TMJOA) is a chronic degenerative joint disease characterized by extracellular matrix (ECM) degradation and chondrocyte apoptosis. The aim of this study was to investigate the role of PRMT1 in TMJOA pathogenesis and its underlying molecular mechanism. Compared to the control group, PRMT1 was highly expressed in IL-1 beta-treated chondrocytes and articular cartilage following MIA injection into rat TMJs. Furthermore, knocking down PRMT1 considerably inhibited ECM degradation and apoptosis induced by IL-1 beta. Mechanistic analyses further revealed that PRMT1 knockdown activated the PI3K/AKT signaling pathway and prevented FOXO1 from translocating to the nucleus. Moreover, an inhibitor of AKT (LY294002) rescued the effect of PRMT1 knockdown on IL-1 beta-induced ECM degradation and apoptosis, and AMI-1, a selective inhibitor of PRMT1, inhibited PRMT1 expression and reversed the pathological progress of TMJOA. Thus, our findings suggest that PRMT1 plays an essential role in ECM degradation and chondrocyte apoptosis in TMJOA via the AKT/FOXO1 signaling pathway.
基金：
Yunnan Provincial Science and Technology Department [202001AY070001-083]; Graduate Innovation Foundation of Kunming Medical University [2020S114]
第一作者机构：[1]Department of Periodontics, The Affiliated Stomatological Hospital of Kunming Medical University, Kunming 650031, Yunnan Province, China[2]Yunnan Key Laboratory of Stomatology, Kunming 650500, Yunnan Province, China[5]The First Dental Clinic of the Affiliated Stomatology Hospital of Kunming Medical University, Kunming 650221, Yunnan Province, China
共同第一作者：
通讯作者：
通讯机构：[3]The First Affiliated Hospital of Kunming Medical University, Kunming 650032, Yunnan Province, China[*1]The First Affiliated Hospital of Kunming Medical University, 295Xichang Road,Kunming 650032, Yunnan Province, China[*2]Department of Dental Research, The affiliated Stomatological Hospital of Kunming Medical University, No. 1088 Mid Hai Yuan Road, Kunming 650500, Yunnan Province, China
推荐引用方式(GB/T 7714)：
Shen Qinhao,Xiao Yiwen,Cheng Bei,et al.PRMT1 promotes extracellular matrix degradation and apoptosis of chondrocytes in temporomandibular joint osteoarthritis via the AKT/ FOXO1 signaling pathway[J].INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY.2021,141:doi:10.1016/j.biocel.2021.106112.
APA：
Shen, Qinhao,Xiao, Yiwen,Cheng, Bei,Sun, Zheyi,Hu, Yu...&Luo, Yingwei.(2021).PRMT1 promotes extracellular matrix degradation and apoptosis of chondrocytes in temporomandibular joint osteoarthritis via the AKT/ FOXO1 signaling pathway.INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY,141,
MLA：
Shen, Qinhao,et al."PRMT1 promotes extracellular matrix degradation and apoptosis of chondrocytes in temporomandibular joint osteoarthritis via the AKT/ FOXO1 signaling pathway".INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY 141.(2021)
