机构：[1]Department of Cardiovascular Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China外科科室普通外科心脏大血管外科(心脏外科)普通外三科(血管外科)昆明医科大学附属第一医院[2]Department of Cardiac and Thoracic Surgery , Third Affiliated Hospital of KunmingMedical University, Kunming, China[3]Department of Anaesthesiology, First Affiliated Hospital of Kunming Medical University, Kunming, China昆明医科大学附属第一医院
BACKGROUND Hemorrhagic shock results in ileal mucosa damage and intestinal bacterial translocation. Additionally, during hemorrhagic shock, norepinephrine levels increase. Past research has shown that the QseC sensor kinase of Escherichia coli modulates the quorum-sensing response to epinephrine and norepinephrine. Therefore, the aim of our study was to examine whether the absence of the ability of E. coli to sense epinephrine/norepinephrine would attenuate the bacterial translocation to extraintestinal organs in a rat model of hemorrhagic shock. METHODS An E. coli MC1000 qseC mutant was constructed, and E. coli MC1000 and MC1000qseC with streptomycin resistance were used to track bacterial translocation after gavage in rats. A rat model of nonlethal hemorrhagic shock was used. The rats were divided into six groups: controls (SS), rats that received a sham shock and MC1000 (M-SS), rats that received a sham shock and MC1000qseC (-SS), rats that received a hemorrhagic shock alone (HS), rats that received a hemorrhagic shock and MC1000 (M-HS), and rats that received a hemorrhagic shock and MC1000qseC (-HS). RESULTS We found the incidence of bacterial translocation in the M-HS rats was higher than in the -HS rats. The observed effects seem to be largely dependent on the inability to sense epinephrine/norepinephrine and the decreased motility of E. coli MC1000qseC. CONCLUSION Therefore, a role for E. coli sensing epinephrine/norepinephrine in the pathophysiology of bacterial translocation following hemorrhagic shock is proposed. The demonstration of such an effect would suggest a new mechanism for the development of shock-induced sepsis.
基金：
National Natural Science Foundation of ChinaNational Natural Science Foundation of China [81560320]; Yunnan Applied Basic research Project [2014FB024]
第一作者机构：[1]Department of Cardiovascular Surgery, First Affiliated Hospital of Kunming Medical University, Kunming, China
通讯作者：
通讯机构：[*1]Department of Anaesthesiology, First Affiliated Hospital of Kunming Medical University, No. 295 XiChang Rd, Kunming City, Yunnan Province, China, 650032
推荐引用方式(GB/T 7714)：
Meng Jun,Huang Yun-chao,Huang Jie,et al.The role of the sensor kinase, QseC, an adrenergic receptor of Escherichia coli, in bacterial translocation during hemorrhagic shock[J].JOURNAL OF TRAUMA AND ACUTE CARE SURGERY.2016,80(6):972-976.doi:10.1097/TA.0000000000001007.
APA：
Meng, Jun,Huang, Yun-chao,Huang, Jie&Yang, Kun.(2016).The role of the sensor kinase, QseC, an adrenergic receptor of Escherichia coli, in bacterial translocation during hemorrhagic shock.JOURNAL OF TRAUMA AND ACUTE CARE SURGERY,80,(6)
MLA：
Meng, Jun,et al."The role of the sensor kinase, QseC, an adrenergic receptor of Escherichia coli, in bacterial translocation during hemorrhagic shock".JOURNAL OF TRAUMA AND ACUTE CARE SURGERY 80..6(2016):972-976
