机构:[1]Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, China[2]Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, China[3]The Third Affiliated Hospital of Kunming Medical University (Yunnan Provincial Tumor Hospital), Kunming, China[4]The First Affiliated Hospital of Kunming Medical University, Kunming, China昆明医科大学附属第一医院[5]Southwest Guizhou Vocational and Technical College for Nationalities, Xingyi, China昆明医科大学附属第一医院
Genetic variations of High-Risk HPV E6/E7 may be associated with the development of cervical cancer in specific geographic regions. Few data have been reported about the HPV prevalence and E6/E7 variants among cervical cancer patients in Southwest China. This study was designed to investigate the prevalence of HPV and E6/E7 variants of most prevalent HPV among cervical cancer patients in Southwest China. After genotyping, E6/E7 genes of most prevalent HR HPV samples were sequenced and analyzed. Phylogenetic trees were then constructed, followed by an analysis of the diversity of secondary structure and selection pressures. HPV 16 (73.8%) and HPV 18 (16.4%) are the most prevalent infection types among cervical cancer patients, followed by HPV 58, HPV 56 and HPV 59, which is different from the high HPV 58 infection rate of outpatients in this region. Eighteen single nucleotide changes were observed in HPV 16 E6 with 13/18 non-synonymous mutations (5 in beta sheet and 2 in alpha helix). Ten single nucleotide changes were identified among HPV 16 E7 with 3/10 non-synonymous mutations. Three single nucleotide changes were observed in HPV 18 E6 with one non-synonymous mutation, and only one synonymous mutation was identified in HPV 18 E7. HPV 16 E6-D25E, E7-N29S and E7-T846C (S95S) exhibited a prevalent linkage mutation. The phylogenetic tree demonstrates that European and Asian lineages were the main patterns. This study may help understand the intrinsic geographical relatedness and oncogenic potential of HR HPV and contributes further to research of diagnostic, therapeutic and therapeutic vaccine strategy. J. Med. Virol. 86:1926-1936, 2014. (c) 2014 Wiley Periodicals, Inc.
基金:
The National Natural Science Foundation of
China; Grant number: 81171946; Grant Sponsor: The Natural
Science Foundation of Yunnan Province; Grant numbers:
2009ZC187M, 2011CA016, 2012FB188; Grant Sponsor: The
Special Research Fund for the Doctoral Program of Higher
Education of China; Grant numbers: 20111106120055;
Grant Sponsor: The Scientific Research Foundation for Returned
Overseas Chinese Scholars, Ministry of Education of China.
第一作者机构:[1]Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, China[2]Yunnan Key Laboratory of Vaccine Research & Development on Severe Infectious Diseases, Kunming, China
通讯作者:
通讯机构:[*1]Institute of Medical Biology, Chinese Academy of Medical Sciences & Peking Union Medical College (CAMS & PUMC), 935 Jiao Ling Road, Kunming, Yunnan Province 650118, China
推荐引用方式(GB/T 7714):
Yang Lijuan,Yang Hongying,Wu Kun,et al.Prevalence of HPV and Variation of HPV 16/HPV 18 E6/E7 Genes in Cervical Cancer in Women in South West China[J].JOURNAL OF MEDICAL VIROLOGY.2014,86(11):1926-1936.doi:10.1002/jmv.24043.
APA:
Yang, Lijuan,Yang, Hongying,Wu, Kun,Shi, Xinan,Ma, Shaohui&Sun, Qiangming.(2014).Prevalence of HPV and Variation of HPV 16/HPV 18 E6/E7 Genes in Cervical Cancer in Women in South West China.JOURNAL OF MEDICAL VIROLOGY,86,(11)
MLA:
Yang, Lijuan,et al."Prevalence of HPV and Variation of HPV 16/HPV 18 E6/E7 Genes in Cervical Cancer in Women in South West China".JOURNAL OF MEDICAL VIROLOGY 86..11(2014):1926-1936