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Role of TEAD4 in colorectal cancer cell proliferation and analysis of its mechanism(Open Access)

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机构： [a]Yunnan Tumor Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China [b]Kunming Medical University Haiyuan College, Kunming, Yunnan, China [c]Department of Gastroenterology, Institute of Gastroenterology, The First Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China [d]HongyunHonghe (Group) Co., Ltd., Kunming, Yunnan, China

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关键词： cell proliferation colorectal cancer TEAD4

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Objective: Colorectal cancer is one of the major causes of tumor-related deaths worldwide. Transcriptional factor TEAD4 is a key factor in the YAP–TAZ signaling pathway. The aim of the present study was to investigate the effect and mechanism of TEAD4 knockdown on colorectal cancer cell proliferation. Methods: Transient transfection and a lentivirus system were used to achieve transient or stable TEAD4 knockdown in Caco2 and HT-29 cells. Western blotting was used to detect transfection efficiency and related protein expression. The sulforhodamine B method and EdU Imaging Kits were used to determine cell viability and DNA synthesis. Results: Western blotting showed that knockdown of TEAD4 could increase the expression of the cell cycle protein p27 in Caco2 (F = 45.78, P < 0.001) and HT-29 (F = 40.71, P < 0.001) cells. Sulforhodamine B assays showed that cell viability with TEAD4 knockdown was significantly decreased in Caco2 and HT-29 cells. Results of EdU assays showed that DNA synthesis was significantly inhibited in Caco2 (Psi#2= 0.001, Psi#3 P < 0.001) and HT-29 cells (Psi#2= 0.001, Psi#3= 0.011) with knockdown of TEAD4. Conclusions: The present study showed that TEAD4 plays an important role in cell proliferation in the Caco2 and HT-29 colorectal cancer cell lines, partially through upregulation of p27, suggesting that TEAD4 is a potential target and biomarker for the development of novel therapeutics for colorectal cancer. © 2018 The Authors. Precision Radiation Oncology published by John Wiley & Sons Australia, Ltd on behalf of Shandong Cancer Hospital & Institute.

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大类 | 4 区医学

小类 | 4 区核医学

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第一作者机构： [a]Yunnan Tumor Hospital, The Third Affiliated Hospital of Kunming Medical University, Kunming, Yunnan, China

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